insertional mutagenesis

1. Insertional mutagenesis is a method for identifying genes by using the integration of DNA as the mutagen, thereby facilitating the cloning of the mutated gene.
   插入突变是一种通过外源DNA整合的方式来获得突变体，并克隆得到对应突变基因的方法。
2. Mutants are generated by chemical treatment, radiation or inserting DNA into the genome ( insertional mutagenesis).
   突变体可以通过化学处理、辐射或在染色体上插入DNA（插入诱变）获得。
3. Insertional mutagenesis using T-DNA, transposons and plasmids are commonly methods to creating a mutant library.
   构建插入突变库的方法有T-DNA插入突变、转座子插入突变和质粒介导的插入突变。
4. In this study, T-DNA insertional mutagenesis and RNA interference ( RNAi) strategies were used to create rice mutants, and functional analysis on corresponding genes and their expression patterns were carried out based on their mutation phenotypes.
   本研究利用T-DNA插入突变和RNA干涉技术创制水稻突变体，并根据突变体的表型来研究相应基因的功能和表达模式。
5. Agrobacterium tumefaciens-mediated transformation of Chaetomium globosum and its T-DNA insertional mutagenesis
   根癌农杆菌介导的球毛壳菌遗传转化及T-DNA插入突变体的获得
6. The mutant strain S.c. Progress in Rice Insertional Mutagenesis
   水稻插入突变库构建研究进展
7. HBV integration is not distributed evenly throughout the host genome and viral insertional mutagenesis may play an important role in the development of HCC.
   结论HBV在肝癌细胞染色体上的整合并不呈均衡分布，病毒对宿主细胞的插入诱变及整合子中持续表达的截短型X蛋白可能在病毒的致癌过程中起重要作用。
8. Insertional mutagenesis has the advantage that the inserted element acts as a tag for gene identification, and it is convenience for both forward and reverse genetics research.
   水稻插入突变体库的建立是功能基因组研究的一个重要内容，在此基础上也能进行正向遗传学及反向遗传学的研究。
9. T-DNA insertional mutagenesis in filamentous fungi and its application prospect
   T-DNA在丝状真菌中的插入突变及其应用前景
10. Insertional Mutagenesis of Trichoderma Obtained by REMI Technique
    利用限制性内切酶技术获得木霉菌插入变异
11. DNA insertional mutagenesis is one of the most effective ways to identify pathogenicity genes of phytopathogenic fungi.
    DNA插入突变是标记、分离植物病原真菌功能基因的有效途径之一。
12. Constructing loss-of-function mutants is one of the most direct and efficient way to study the function of gene. While insertional mutagenesis is the easiest strategy, resources based on mutations caused by defined DNA insertions are likely to have an enduring importance in functional genomics.
    在功能基因组学中，创建基因的功能缺失突变体是研究目的基因功能的最直接和有效的途径，转座子插入诱变因为在基因敲除上具有极大的优势而受到了广泛地运用。

noun

1. (genetics) a mutation caused by the insertion of exogenous DNA into a genome